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Promega T7 RNA Polymerase

$1,796.00

Unit: Each of 1

Description

  • DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (
  • Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates

Specifications

Concentration 80 U/μL
Components 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
pH 7.7
Storage Buffer 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton X-100, 50% Glycerol
Source Recombinant E. coli strain
Product Type T7 RNA Polymerase
Form Liquid
Color Colorless
Purity >90%
For Use With (Application) Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
Content And Storage -30°C to -10°C
Quantity 10,000 U
Inhibitors Recombinant RNasin Ribonuclease

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