Description
- DNA-Dependent RNA Polymerase Specific for T7 Promoter Sequences
- >90% pure as determined by SDS polyacrylamide gel electrophoresis
- Free of detectable levels of contaminating RNase and DNase activity (
- Incorporates 32P, 33P, 3H and 35S nucleoside triphosphates
Specifications
| Concentration | 80 U/μL |
| Components | 5X Reaction Buffer: 100mM DTT and transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl |
| pH | 7.7 |
| Storage Buffer | 20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton X-100, 50% Glycerol |
| Source | Recombinant E. coli strain |
| Product Type | T7 RNA Polymerase |
| Form | Liquid |
| Color | Colorless |
| Purity | >90% |
| For Use With (Application) | Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA |
| Content And Storage | -30°C to -10°C |
| Quantity | 10,000 U |
| Inhibitors | Recombinant RNasin Ribonuclease |
