Promega SRC Kinase Enzyme System

Description

Includes

SRC Kinase Assay:

• Kinase: Recombinant Human SRC, 10μg (full length), MW: ≅83kDa
• Substrate: SRC substrate (KVEKIGEGTYGVVYK-amide) derived from human p34cdc2 (amino acid 6 to 20)
• Other: Reaction Buffer, DTT, MnCl2

ADP-Glo Kinase Assay: Reagent, buffer, substrate, Ultra Pure ATP (10mM), ADP (10mM)

Recombinant full-length human SRC was expressed in E. coli cells using an N-terminal GST tag. SRC was originally identified as a transforming protein of the Rous sarcoma virus that had enzymatic ability to phosphorylate tyrosine in protein substrates.

• Easily Screen and Profile SRC Kinase Inhibitors
• Includes kinase, substrate and reaction buffer
• Use with ADP-Glo Assay for bioluminescent detection of kinase activity