Description
Histone H3 Monoclonal specifically detects Histone H3 in Human, Mouse, Bovine, Hamster samples. It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Microarray.
Specifications
| Antigen | Histone H3 (p Ser28) |
| Classification | Monoclonal |
| Concentration | 0.5 mg/mL |
| Dilution | Western Blot 0.5-1.0 ug/ml, Flow Cytometry 1:10-1:1000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:2000, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Microarray |
| Gene Alias | H3, H3 histone, family 3A, H3.3A, H3.3B, H3F2, H3F3, H3F3B, H3FM, H3S28p, HIST2H3C, histone H3.3, MGC87782, MGC87783 |
| Host Species | Rat |
| Molecular Weight of Antigen | 15 kDa |
| Quantity | 0.1 mL |
| Research Discipline | Chromatin Modifiers, Chromatin Research, Epigenetics, Histones and Modified Histones, Loading Controls |
| Gene ID (Entrez) | 126961 |
| Target Species | Human, Mouse, Bovine, Hamster |
| Isotype | IgG2a |
| Applications | Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin) |
| Clone | HTA28 |
| Conjugate | Unconjugated |
| Gene Accession No. | P84243 |
| Gene Symbols | H3C14 |
| Immunogen | This Histone H3 [p Ser28] antibody (HTA28) was raised against synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 (pSer28) of Human Histone H3. |
| Purification Method | Affinity Purified |
| Regulatory Status | RUO |
| Primary or Secondary | Primary |
| Test Specificity | Histone H3 [p Ser28] antibody (HTA28) does not detect the unphosphorylated epitope. It detects the phosphorylated histone molecule at the onset of mitosis (prophase, metaphase and weaker at the beginning of anaphase), but not during late anaphase. |
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
