Description
Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/mL. The His tag is a short amino acid sequence consists of Histidine (His) residues appended to recombinant proteins. Ni-charged resins are used for the purification of His-tagged proteins from eukaryotic and prokaryotic expression systems. An anti-His tag antibody is a useful tool for the analysis of His-tagged proteins.THE His Tag Antibody [iFluor 647], mAb, Mouse is THE His Tag Antibody (A00186) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of His-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
His Tag Monoclonal antibody specifically detects His Tag in Tag samples. It is validated for Flow Cytometry, Immunocytochemistry
Specifications
| Antigen | His Tag |
| Classification | Monoclonal |
| Conjugate | iFluor 647 |
| Gene Alias | 6His tag; 6X His; His Tag; Histidine Tag |
| Immunogen | A synthetic peptide HHHHHH coupled to KLH |
| Quantity | 100 μg |
| Primary or Secondary | Primary |
| Product Type | Antibody |
| Isotype | IgG1 κ |
| Applications | Flow Cytometry, Immunocytochemistry |
| Clone | 6G2A9 |
| Formulation | PBS with 10mg/mL BSA and 0.02% sodium azide; pH 7.4 |
| Host Species | Mouse |
| Purification Method | Protein A |
| Regulatory Status | RUO |
| Content And Storage | -20°C or -80°C if preferred |
| Form | Lyophilized |
| Target Molecule | Tag |
